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Journal: Antibiotics
Article Title: Evaluation of the Inactivation of Microorganisms by a Blue Laser (445 nm)—An In Vitro Study
doi: 10.3390/antibiotics15020137
Figure Lengend Snippet: A box plot of the log 10 -transformed colony-forming unit (CFU) values of Enterococcus faecalis , Streptococcus mutans , and Candida albicans in untreated samples (negative controls), laser-treated samples (test groups 4 mm/s, 2 mm/s, and 1 mm/s), and positive controls treated with chlorhexidine digluconate (CHX). Data source .
Article Snippet: The
Techniques: Transformation Assay
Journal: Antibiotics
Article Title: Evaluation of the Inactivation of Microorganisms by a Blue Laser (445 nm)—An In Vitro Study
doi: 10.3390/antibiotics15020137
Figure Lengend Snippet: Testing of the carrier solutions: phosphate buffered saline (PBS), deionised H 2 O, potassium phosphate buffer (KPO 4 ), sodium potassium phosphate buffer (NaKPO 4 ) and Tris-buffered saline with hydrochloric acid (Tris-HCl) by applying the microorganisms to the ceramic test specimens, vital staining using MTT, and subsequent microscopic evaluation of the microbial converted blue formazan. Tris-HCl reduced the viability slightly for Enterococcus faecalis and strongly for Streptococcus mutans . Sodium potassium phosphate buffer was not suitable for any of the test organisms. PBS was also not optimal for this test setup, with clearly recognisable salt crystals forming. Stable viability results were shown with the H 2 O (deionised) solution for Candida albicans and Enterococcus faecalis and with the potassium phosphate buffer solution for Streptococcus mutans . Figure source .
Article Snippet: The
Techniques: Saline, Staining